Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences. T7 lysozyme expression suppresses basal T7 expression.
BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. T7 lysozyme expression suppresses basal T7 expression.
Origami B host strain derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG. T7 lysozyme expression suppresses basal T7 expression.
Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.
Rosetta-gami B strains combines the key features of BL21, Origami, and Rosetta to enhances the expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.
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NovaBlue GigaSingles™ Competent Cells produce greater than 1 x 10⁹ colonies/μg plasmid DNA for cloning applications requiring high-efficiency transformations.